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AP Biology · Unit 3: Cellular Energetics

3.5 Photosynthesis

Turning light into sugar

Photosynthesis converts light energy into chemical energy (glucose). The overall equation: 6 CO₂ + 6 H₂O + light → C₆H₁₂O₆ + 6 O₂. It happens in chloroplasts, where the pigment chlorophyll absorbs light. The oxygen released comes from splitting water.

Two stages

The light-dependent reactions occur in the thylakoid membranes: they split water, release O₂, and make ATP and NADPH. The Calvin cycle (light-independent) occurs in the stroma: it uses that ATP and NADPH to fix CO₂ into sugar (G3P → glucose).

Key terms

Photosynthesis
Converting light energy into chemical energy (sugar): 6CO₂ + 6H₂O + light → C₆H₁₂O₆ + 6O₂.
Light-dependent reactions
In the thylakoid membranes: split water, release O₂, make ATP and NADPH.
Calvin cycle
In the stroma: uses ATP and NADPH to fix CO₂ into sugar.
Chlorophyll
The green pigment that absorbs light energy for photosynthesis.
NADPH
An electron carrier made in the light reactions that powers the Calvin cycle.

Try an AP-style question

Experiment

Plants are given water labeled with the heavy oxygen isotope ¹⁸O, and normal CO₂.

Where will the ¹⁸O appear?

  1. In the glucose produced by the Calvin cycle
  2. In the CO₂ released by respiration
  3. In the ATP made by the thylakoids
  4. In the O₂ released by the plant
Show the answer

D. O₂ comes from splitting water at photosystem II; the oxygen in sugars comes from CO₂.

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